Civic Intelligence

Two Frontiers Project Inc.

990 • Fiscal year 2024 • EIN 99-2011215

Mar 19, 2024 to Dec 31, 2024 • Filed on Oct 28, 2025

Organization ProfileWWW.TWOFRONTIERS.ORG

Refreshing map…

3336 Dudley WayFort Collins, CO 80526

(781) 626-2626

Siviq Scores

Precomputed percentiles for this filing year versus similar nonprofits in the same peer cohort.

Liabilities / Assets

48th percentile

0.01x

Higher debt load relative to assets than 48% of similar nonprofits.

2024 filings • 501(c)3 • $500k-$1M nonprofits • Source year 2024

Liabilities / Revenue

42nd percentile

0.01x

Higher debt load relative to revenue than 42% of similar nonprofits.

2024 filings • 501(c)3 • $500k-$1M nonprofits • Source year 2024

Net Margin

91st percentile

52%

Higher net margin than 91% of similar nonprofits.

2024 filings • 501(c)3 • $500k-$1M nonprofits • Source year 2024

Top Officer Pay

56th percentile

$5,770

Higher top officer pay than 56% of similar nonprofits.

Top officer pay equals 1.1% of source-year revenue.

2024 filings • 501(c)3 • $500k-$1M nonprofits • Source year 2024

Asset Growth

Score unavailable

No value available

No earlier valid filing was available within the previous three public years.

Source year 2024

Revenue Growth

Score unavailable

No value available

No earlier valid filing was available within the previous three public years.

Source year 2024

Balance Sheet
Liabilities / Assets48th percentile

Assets

$285,745

No earlier filing loaded for comparison.

Net Assets

$282,355

No earlier filing loaded for comparison.

Liabilities

$3,390

No earlier filing loaded for comparison.

Revenue And Expenses
Liabilities / Revenue42nd percentileNet Margin91st percentile

Revenue

$538,377

No earlier filing loaded for comparison.

Expenses

$255,772

No earlier filing loaded for comparison.

Net Income

$282,605

No earlier filing loaded for comparison.

Historical Trend

Balance Sheet Trend

Asset GrowthScore unavailable

The highlighted filing sits inside the broader history for assets, liabilities, and net assets.

$300K$200K$100K$0Assets 2024: $285,745Liabilities 2024: $3,390Net Assets 2024: $282,3552024

Highlighted filing

2024

Assets$285,745
Liabilities$3,390
Net Assets$282,355
Open 2024 Filing

Operations Trend

Revenue GrowthScore unavailable

Revenue, expenses, and net income across loaded years, with this filing highlighted.

$600K$400K$200K$0Revenue 2024: $538,377Expenses 2024: $255,772Net Income 2024: $282,6052024

Highlighted filing

2024

Revenue$538,377
Expenses$255,772
Net Income$282,605
Open 2024 Filing
Jump To
Filing Snapshot
Filing Period
Mar 19, 2024 to Dec 31, 2024
Signed
Oct 28, 2025
Return Version
2024v5.2
Gross Receipts
$538,377
Mission and Program Overview

Mission

The principal purpose of two frontiers is to advance humanity's understanding of life's adaptation to extreme environments through scientific research and education. The organization plans and leads scientific expeditions to remote oceanic, terrestrial, and other high-stress environments to study how microorganisms survive and influence natural systems.using modern sequencing and microbiology tools, two frontiers collects, analyzes, and shares data to support environmental recovery and contamination mitigation. Its open-access databases, software, and publications are freely available to researchers for non-commercial purposes. The organization may also fund academic or nonprofit partners to conduct related scientific studies that contribute to public knowledge.

To explore, understand, and harness microbial life-from sea to space.

Balance Sheet Detail
LineBeginningEndChange
Assets
Cash and Non-Interest-Bearing Accounts$0$265,045▲ $265,045
Land, Buildings, and Equipment, Net$0$20,700▲ $20,700
Total Assets$0$285,745▲ $285,745
Liabilities
Deferred Revenue$0$3,390▲ $3,390
Total Liabilities$0$3,390▲ $3,390
Net Assets / Fund Balance
Net Assets Without Donor Restrictions$0$282,355▲ $282,355
Total Net Assets Fund Balance$0$282,355▲ $282,355
Total Liabilities and Net Assets / Fund Balance$0$285,745▲ $285,745

Asset Categories

AssetBook ValueDepreciationBasis
Equipment$20,700$2,300$23,000
Compensation and Service Providers
Top Officer Pay56th percentile

Employees

NameTitleFull / Part TimeBaseOtherTotal
Krista RyonTreasurer and Director of OperationsFT$4,263$1,507$5,770
Braden TierneyPresident and Executive DirectorFT-$1,510$1,510

Board Members and Trustees

NameTitle
Eric WardDirector
James HenriksenSecretary and Director of Research and Development
Revenue and Support

Revenue Composition

Contributions and Grants
$538,219
Program Service Revenue
$0
Investment Income
$0
Other Revenue
$158
All Other Contributions
$538,219
Change in Net Assets
$282,605

Noncash Contribution Practices

Property subject to holding requirements
No
Reviewed unusual noncash gifts
No
Third parties used for noncash contributions
No

Noncash Contributions

Contribution TypeContribution CountReported AmountValuation Method
Other Non Cash Contri Table1$123,464Cost
Total Noncash Contributions1$123,464-
Expenses and Functional Allocation

Major Expense Lines

Line ItemAmount
Other Expenses$192,857
Grants and Similar Amounts Paid$50,000
Salaries, Compensation, and Employee Benefits$12,915
Total Fundraising Expense$1,623
Professional Fundraising Fees$0

Functional Expense Allocation

Line ItemProgramManagementFundraisingTotal
Grants to Domestic Orgs$50,000--$50,000
Travel$28,898$4,698-$33,596
Fees for Services Other$28,173$20$10$28,203
Current Officers, Directors, Trustees, and Key Employees$8,146$2,509$1,519$12,174
Fees for Services Accounting-$7,340-$7,340
Office Expenses$773$3,414$2$4,189
Conferences and Meetings$3,209--$3,209
Depreciation Depletion-$2,300-$2,300
Information Technology$1,954$340-$2,294
Other Expenses$1,017$249-$1,017
Payroll Taxes$496$153$92$741
Fees for Services Legal-$550-$550
Advertising$352--$352
Total Functional Expenses$232,576$21,573$1,623$255,772
International Activity

Grant and Assistance Recipients

RecipientLocationCategoryPurposeAmount
Colorado State UniversityFort Collins, COGovSUPPORT THE ONGOING EFFORTS OF NREL'S CULTUROMICS TEAM$50,000
Fundraising, Events, and Gaming
Fundraising activities
No
Gaming activities
No
Professional fundraiser used
No

Fundraising and Gaming Totals

Line ItemAmount
Professional Fundraising Fees$0
Political and Lobbying Activity
Political campaign activity
No
Lobbying activity
No
Subject to proxy tax
No
Governance and Compliance

Governance Checklist

Compiled or reviewed by an accountant
No
Annual disclosure for covered persons
Yes
Business relationship with 35% controlled entity
No
Business relationship with family members
No
Business relationship with organization members
No
Material changes to governing documents
No
Compensation from other sources disclosed
No
CEO compensation reviewed
No
Other officer compensation reviewed
No
Conflict-of-interest policy
Yes
Audited financial statements prepared
No
Key decisions subject to board approval
No
Management duties delegated
No

Governance Explanations

Form 990, Part VI, Section B, Line 11B

“The form 990 was provided to the organization's governing body before it was filed with the irs.”

Form 990, Part VI, Section B, Line 12C

“Potential conflicts of interest are discussed in board meetings and any members with an active conflict of interest are recused during relevant sections of the board meetings.”

Form 990, Part VI, Section C, Line 19

“The organization makes its governing documents, conflict of interest policy, and financial statements available to the public upon request.”

Filing and Contact Details

Filer

Filer Name
The Two Frontiers Project Inc
EIN
99-2011215
Phone
7816262626
Address
3336 DUDLEY WAY, FORT COLLINS, CO 80526

Signing Officer

Name
Braden Tierney
Title
Executive Director
Phone
7816262626
Signed
2025-10-28
Discuss with paid preparer
Yes

Organization Details

Principal Officer
Braden Tierney
Formed
2024
Legal Domicile
De
Voting Board Members
4
Independent Board Members
2
Employees
1
Volunteers
4

Preparer

Firm
Wegner Cpas Llp
Address
419 N LEE ST, ALEXANDRIA, VA 22314-2301
Preparer
Adam Kuczynski CPA
Phone
7035190990
Supplemental Narrative

Additional Explanations

Form 990, Part IX, Line 11G

“Other professional fees: program service expenses 28,111. Management and general expenses 0. Fundraising expenses 0. Total expenses 28,111. Payroll processing fees: program service expenses 62. Management and general expenses 20. Fundraising expenses 10. Total expenses 92.”

FORM 990, PART XI, LINE 9:

“Foreign currency exchange gain/loss -250.”

Raw XML Appendix368 raw XML fields

This appendix keeps the raw XML leaves available for debugging and edge-case review. The human report above is the primary experience.

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IRS990/Desc0IN 2024, THE TWO FRONTIERS PROJECT ADVANCED ITS CARBON INITIATIVE, FOCUSED ON DISCOVERING AND CHARACTERIZING NOVEL MICROORGANISMS THAT NATURALLY CAPTURE AND RECYCLE CARBON. THE TEAM CONDUCTED FIELDWORK IN JAPAN AND PAPUA NEW GUINEA, ISOLATING AND ANALYZING CYANOBACTERIA SUCH AS THE NEWLY IDENTIFIED CHONKUS LINEAGE CAPABLE OF ENHANCED CARBON FIXATION IN EXTREME ENVIRONMENTS. THESE DISCOVERIES SUPPORTED THE DEVELOPMENT OF A NEW LABORATORY AND DATA PLATFORM FOR ASSESSING MICROBIAL CARBON-CAPTURE POTENTIAL. THE ORGANIZATION ALSO LAUNCHED COMPLEMENTARY CITIZEN SCIENCE PROGRAMS, INCLUDING "IN YOUR HOME AND "IN THE WILD", WHICH TRAINED MEMBERS OF THE PUBLIC TO COLLECT ENVIRONMENTAL SAMPLES FOR ANALYSIS. EDUCATIONAL WORKSHOPS ACCOMPANYING THESE PROGRAMS INTRODUCED PARTICIPANTS TO MICROBIOLOGY, CULTURING TECHNIQUES, AND THE ROLE OF MICROORGANISMS IN MITIGATING POLLUTION AND MAINTAINING ECOSYSTEM BALANCE.
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IRS990/MissionDesc0THE PRINCIPAL PURPOSE OF TWO FRONTIERS IS TO ADVANCE HUMANITY'S UNDERSTANDING OF LIFE'S ADAPTATION TO EXTREME ENVIRONMENTS THROUGH SCIENTIFIC RESEARCH AND EDUCATION. THE ORGANIZATION PLANS AND LEADS SCIENTIFIC EXPEDITIONS TO REMOTE OCEANIC, TERRESTRIAL, AND OTHER HIGH-STRESS ENVIRONMENTS TO STUDY HOW MICROORGANISMS SURVIVE AND INFLUENCE NATURAL SYSTEMS.USING MODERN SEQUENCING AND MICROBIOLOGY TOOLS, TWO FRONTIERS COLLECTS, ANALYZES, AND SHARES DATA TO SUPPORT ENVIRONMENTAL RECOVERY AND CONTAMINATION MITIGATION. ITS OPEN-ACCESS DATABASES, SOFTWARE, AND PUBLICATIONS ARE FREELY AVAILABLE TO RESEARCHERS FOR NON-COMMERCIAL PURPOSES. THE ORGANIZATION MAY ALSO FUND ACADEMIC OR NONPROFIT PARTNERS TO CONDUCT RELATED SCIENTIFIC STUDIES THAT CONTRIBUTE TO PUBLIC KNOWLEDGE.
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IRS990/ProgSrvcAccomActy2Grp/Desc0THROUGH ITS CROPS INITIATIVE, THE TWO FRONTIERS PROJECT BEGAN BUILDING A RESEARCH PLATFORM TO IDENTIFY AND CULTURE MICROORGANISMS THAT ENHANCE AGRICULTURAL PRODUCTIVITY, WATER RETENTION, AND PLANT RESILIENCE UNDER DROUGHT AND NUTRIENT STRESS. IN 2024, THE ORGANIZATION CONDUCTED AN EXPEDITION TO THE MOJAVE DESERT TO STUDY NATURALLY STRESS-ADAPTED MICROBIAL COMMUNITIES ASSOCIATED WITH DESERT FLORA AND SOIL CRUSTS. THESE FINDINGS INFORMED THE DESIGN OF FUTURE EXPERIMENTS FOCUSED ON BIOLOGICAL SUPPORT SYSTEMS FOR REGENERATIVE AGRICULTURE. CONCURRENTLY, 2FP EXPANDED ITS LABORATORY INFRASTRUCTURE FOR MICROBIAL ISOLATION, CULTURE, AND FUNCTIONAL TESTING, LAYING THE GROUNDWORK FOR A SCALABLE DATABASE AND OPEN TOOLKIT FOR ENVIRONMENTAL AND CROP-ASSOCIATED MICROBIOME RESEARCH.
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IRS990ScheduleD/TotalBookValueLandBuildingsAmt020700
IRS990ScheduleI/GrantRecordsMaintainedInd00
IRS990ScheduleI/RecipientTable/CashGrantAmt050000
IRS990ScheduleI/RecipientTable/IRCSectionDesc0GOV
IRS990ScheduleI/RecipientTable/NonCashAssistanceAmt00
IRS990ScheduleI/RecipientTable/PurposeOfGrantTxt0SUPPORT THE ONGOING EFFORTS OF NREL'S CULTUROMICS TEAM
IRS990ScheduleI/RecipientTable/RecipientBusinessName/BusinessNameLine1Txt0COLORADO STATE UNIVERSITY
IRS990ScheduleI/RecipientTable/RecipientEIN0846000545
IRS990ScheduleI/RecipientTable/USAddress/AddressLine1Txt01060 WARNER STREET
IRS990ScheduleI/RecipientTable/USAddress/CityNm0FORT COLLINS
IRS990ScheduleI/RecipientTable/USAddress/StateAbbreviationCd0CO
IRS990ScheduleI/RecipientTable/USAddress/ZIPCd080523
IRS990ScheduleI/SupplementalInformationDetail/ExplanationTxt0THE ORGANIZATION DOES NOT HAVE ANY FORMAL PROCEDURES IN PLACE TO MONITOR THE USE OF GRANT FUNDS.
IRS990ScheduleI/SupplementalInformationDetail/FormAndLineReferenceDesc0PART I, LINE 2:
IRS990ScheduleI/Total501c3OrgCnt01
IRS990ScheduleI/TotalOtherOrgCnt00
IRS990/ScheduleJRequiredInd00
IRS990ScheduleM/AnyPropertyThatMustBeHeldInd00
IRS990ScheduleM/Form8283ReceivedCnt00
IRS990ScheduleM/OtherNonCashContriTableGrp/ContributionCnt01
IRS990ScheduleM/OtherNonCashContriTableGrp/Desc0FREEZERS AND LAB EQUIPMENT
IRS990ScheduleM/OtherNonCashContriTableGrp/MethodOfDeterminingRevenuesTxt0COST
IRS990ScheduleM/OtherNonCashContriTableGrp/NonCashCheckboxInd0X
IRS990ScheduleM/OtherNonCashContriTableGrp/NoncashContributionsRptF990Amt0123464
IRS990ScheduleM/ReviewProcessUnusualNCGiftsInd00
IRS990ScheduleM/SupplementalInformationDetail/ExplanationTxt0THE NUMBER OF CONTRIBUTIONS LISTED ABOVE IS THE NUMBER OF CONTRIBUTIONS MADE. ALL FREEZERS AND LAB EQUIPMENT WERE DONATED FROM ONE CONTRIBUTOR ON MULTIPLE DATES.
IRS990ScheduleM/SupplementalInformationDetail/FormAndLineReferenceDesc0PART I, COLUMN (B):
IRS990ScheduleM/ThirdPartiesUsedInd00
IRS990/ScheduleORequiredInd01
IRS990ScheduleO/SupplementalInformationDetail/ExplanationTxt0THE FORM 990 WAS PROVIDED TO THE ORGANIZATION'S GOVERNING BODY BEFORE IT WAS FILED WITH THE IRS.
IRS990ScheduleO/SupplementalInformationDetail/ExplanationTxt1POTENTIAL CONFLICTS OF INTEREST ARE DISCUSSED IN BOARD MEETINGS AND ANY MEMBERS WITH AN ACTIVE CONFLICT OF INTEREST ARE RECUSED DURING RELEVANT SECTIONS OF THE BOARD MEETINGS.
IRS990ScheduleO/SupplementalInformationDetail/ExplanationTxt2THE ORGANIZATION MAKES ITS GOVERNING DOCUMENTS, CONFLICT OF INTEREST POLICY, AND FINANCIAL STATEMENTS AVAILABLE TO THE PUBLIC UPON REQUEST.
IRS990ScheduleO/SupplementalInformationDetail/ExplanationTxt3OTHER PROFESSIONAL FEES: PROGRAM SERVICE EXPENSES 28,111. MANAGEMENT AND GENERAL EXPENSES 0. FUNDRAISING EXPENSES 0. TOTAL EXPENSES 28,111. PAYROLL PROCESSING FEES: PROGRAM SERVICE EXPENSES 62. MANAGEMENT AND GENERAL EXPENSES 20. FUNDRAISING EXPENSES 10. TOTAL EXPENSES 92.
IRS990ScheduleO/SupplementalInformationDetail/ExplanationTxt4FOREIGN CURRENCY EXCHANGE GAIN/LOSS -250.
IRS990ScheduleO/SupplementalInformationDetail/FormAndLineReferenceDesc0FORM 990, PART VI, SECTION B, LINE 11B
IRS990ScheduleO/SupplementalInformationDetail/FormAndLineReferenceDesc1FORM 990, PART VI, SECTION B, LINE 12C
IRS990ScheduleO/SupplementalInformationDetail/FormAndLineReferenceDesc2FORM 990, PART VI, SECTION C, LINE 19
IRS990ScheduleO/SupplementalInformationDetail/FormAndLineReferenceDesc3FORM 990, PART IX, LINE 11G
IRS990ScheduleO/SupplementalInformationDetail/FormAndLineReferenceDesc4FORM 990, PART XI, LINE 9:
IRS990/SchoolOperatingInd00
IRS990/SignificantChangeInd00
IRS990/SignificantNewProgramSrvcInd00
IRS990/SubjectToExcsTaxNetInvstIncInd00
IRS990/SubjectToProxyTaxInd00
IRS990/SubjToTaxRmnrtnExPrchtPymtInd00
IRS990/TaxablePartyNotificationInd00
IRS990/TaxExemptBondsInd00
IRS990/TerminateOperationsInd00
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IRS990/TotalAssetsGrp/BOYAmt00
IRS990/TotalAssetsGrp/EOYAmt0285745
IRS990/TotalCompGreaterThan150KInd00
IRS990/TotalContributionsAmt0538219
IRS990/TotalEmployeeCnt01
IRS990/TotalFunctionalExpensesGrp/FundraisingAmt01623
IRS990/TotalFunctionalExpensesGrp/ManagementAndGeneralAmt021573
IRS990/TotalFunctionalExpensesGrp/ProgramServicesAmt0232576
IRS990/TotalFunctionalExpensesGrp/TotalAmt0255772
IRS990/TotalGrossUBIAmt00
IRS990/TotalLiabilitiesEOYAmt03390
IRS990/TotalLiabilitiesGrp/BOYAmt00
IRS990/TotalLiabilitiesGrp/EOYAmt03390
IRS990/TotalNetAssetsFundBalanceGrp/BOYAmt00
IRS990/TotalNetAssetsFundBalanceGrp/EOYAmt0282355
IRS990/TotalOtherCompensationAmt03017
IRS990/TotalOtherProgSrvcExpenseAmt05003
IRS990/TotalOtherProgSrvcGrantAmt00
IRS990/TotalOtherProgSrvcRevenueAmt00
IRS990/TotalProgramServiceExpensesAmt0232576
IRS990/TotalReportableCompFromOrgAmt04263
IRS990/TotalRevenueGrp/ExclusionAmt0158
IRS990/TotalRevenueGrp/RelatedOrExemptFuncIncomeAmt00
IRS990/TotalRevenueGrp/TotalRevenueColumnAmt0538377
IRS990/TotalRevenueGrp/UnrelatedBusinessRevenueAmt00
IRS990/TotalVolunteersCnt04
IRS990/TotLiabNetAssetsFundBalanceGrp/BOYAmt00
IRS990/TotLiabNetAssetsFundBalanceGrp/EOYAmt0285745
IRS990/TotReportableCompRltdOrgAmt00
IRS990/TravelGrp/ManagementAndGeneralAmt04698
IRS990/TravelGrp/ProgramServicesAmt028898
IRS990/TravelGrp/TotalAmt033596
IRS990/TrnsfrExmptNonChrtblRltdOrgInd00
IRS990/TypeOfOrganizationCorpInd0X
IRS990/UnrelatedBusIncmOverLimitInd00
IRS990/UponRequestInd0X
IRS990/USAddress/AddressLine1Txt03336 DUDLEY WAY
IRS990/USAddress/CityNm0FORT COLLINS
IRS990/USAddress/StateAbbreviationCd0CO
IRS990/USAddress/ZIPCd080526
IRS990/VotingMembersGoverningBodyCnt04
IRS990/VotingMembersIndependentCnt02
IRS990/WebsiteAddressTxt0WWW.TWOFRONTIERS.ORG
IRS990/WhistleblowerPolicyInd00
ReturnHeader/BuildTS02025-03-06 01:10:19Z
ReturnHeader/BusinessOfficerGrp/DiscussWithPaidPreparerInd01
ReturnHeader/BusinessOfficerGrp/PersonNm0BRADEN TIERNEY
ReturnHeader/BusinessOfficerGrp/PersonTitleTxt0EXECUTIVE DIRECTOR
ReturnHeader/BusinessOfficerGrp/PhoneNum07816262626
ReturnHeader/BusinessOfficerGrp/SignatureDt02025-10-28
ReturnHeader/Filer/BusinessName/BusinessNameLine1Txt0THE TWO FRONTIERS PROJECT INC
ReturnHeader/Filer/BusinessNameControlTxt0TWOF
ReturnHeader/Filer/EIN0992011215
ReturnHeader/Filer/PhoneNum07816262626
ReturnHeader/Filer/USAddress/AddressLine1Txt03336 DUDLEY WAY
ReturnHeader/Filer/USAddress/CityNm0FORT COLLINS
ReturnHeader/Filer/USAddress/StateAbbreviationCd0CO
ReturnHeader/Filer/USAddress/ZIPCd080526
ReturnHeader/IRSResponsiblePrtyInfoCurrInd01
ReturnHeader/PreparerFirmGrp/PreparerFirmEIN0390974031
ReturnHeader/PreparerFirmGrp/PreparerFirmName/BusinessNameLine1Txt0WEGNER CPAS LLP
ReturnHeader/PreparerFirmGrp/PreparerUSAddress/AddressLine1Txt0419 N LEE ST
ReturnHeader/PreparerFirmGrp/PreparerUSAddress/CityNm0ALEXANDRIA
ReturnHeader/PreparerFirmGrp/PreparerUSAddress/StateAbbreviationCd0VA
ReturnHeader/PreparerFirmGrp/PreparerUSAddress/ZIPCd0223142301
ReturnHeader/PreparerPersonGrp/PhoneNum07035190990
ReturnHeader/PreparerPersonGrp/PreparationDt02025-10-28
ReturnHeader/PreparerPersonGrp/PreparerPersonNm0ADAM KUCZYNSKI CPA
ReturnHeader/ReturnTs02025-10-29T10:34:09-05:00
ReturnHeader/ReturnTypeCd0990
ReturnHeader/TaxPeriodBeginDt02024-03-19
ReturnHeader/TaxPeriodEndDt02024-12-31
ReturnHeader/TaxYr02024

Document Assets

No mirrored PDF or thumbnail assets are attached yet.

Filings

Displayed year

2024 • Form 990Detailed filing. Detailed filing data is available for this year.

Open filing
Assets
$285,745
Liabilities
$3,390
Net assets
$282,355
Revenue
$538,377
Expenses
$255,772
Net income
$282,605
Balance SheetOperations
YearAssetsLiabilitiesNet AssetsRevenueExpensesNet Income
2024Detailed filing. Detailed filing data is available for this year.$2.86$0.03$2.82$5.38$2.56$2.83